How to reconstitute GLOW
GLOW is a three-peptide blend sold as one 70 mg vial: 10 mg BPC-157, 10 mg TB-500 and 50 mg GHK-Cu. Every amount you draw holds the three in the same 1 : 1 : 5 ratio, so the chart gives the total and each peptide's share. The mixed liquid turns blue from the copper in GHK-Cu. Labels differ between makers; this chart is for the 10/10/50 split, and the calculator takes any other.
- 70 mg (10/10/50) vial: add 3 mL of bacteriostatic water. That makes 23.33 mg/mL, so each unit on a U-100 insulin syringe holds 233.3 mcg, and 2.8 mg is 12 units.
Get this chart as a printable PDF
The amounts are examples for the arithmetic, not recommendations. For research and education only.
Matthew’s GLOW mixing video is coming to Reconstitution Insights.
GLOW units chart
What each amount comes to on a 1 mL (U-100) insulin syringe, with the water used in the video. Using a different amount of water? The calculator works it out.
70 mg (10/10/50) vial + 3 mL bacteriostatic water
23.33 mg/mL concentration233.3 mcg in each unit
| Amount | Units (U-100) | Volume | Of which |
|---|---|---|---|
| 1.4 mg200 mcg BPC-157 200 mcg TB-500 1 mg GHK-Cu | 6 units | 0.06 mL | 200 mcg BPC-157 200 mcg TB-500 1 mg GHK-Cu |
| 2.1 mg300 mcg BPC-157 300 mcg TB-500 1.5 mg GHK-Cu | 9 units | 0.09 mL | 300 mcg BPC-157 300 mcg TB-500 1.5 mg GHK-Cu |
| 2.8 mg400 mcg BPC-157 400 mcg TB-500 2 mg GHK-Cu | 12 units | 0.12 mL | 400 mcg BPC-157 400 mcg TB-500 2 mg GHK-Cu |
| 3.5 mg500 mcg BPC-157 500 mcg TB-500 2.5 mg GHK-Cu | 15 units | 0.15 mL | 500 mcg BPC-157 500 mcg TB-500 2.5 mg GHK-Cu |
| 4.2 mg600 mcg BPC-157 600 mcg TB-500 3 mg GHK-Cu | 18 units | 0.18 mL | 600 mcg BPC-157 600 mcg TB-500 3 mg GHK-Cu |
Step by step
The same steps as the video, in writing.
- Check the label.
The vial says how much powder is in it: 70 mg (10/10/50). For a blend, the label gives the total and the split: BPC-157 + TB-500 + GHK-Cu.
- Draw the water.
Use bacteriostatic water: 3 mL for the 70 mg (10/10/50) vial. If it's more than 1 mL, a 3 mL syringe does it in one draw.
- Add it slowly.
Let the water run down the inside wall of the vial, then swirl gently until the powder has dissolved. Don't shake it.
- Write the strength on the vial.
For the 70 mg (10/10/50) vial that's 23.33 mg/mL, or 233.3 mcg per unit, plus the date you mixed it.
- Find your amount.
Read the units from the chart, or put your own numbers in the calculator. Then check it backwards: units × mcg per unit should give the amount you wanted.
On Peptide Insights
The GLOW overview
For what GLOW is and what research has looked at, here is Matthew's full video on Peptide Insights, his main channel. 11:42 long.
Free printable PDF
Get the GLOW chart as a printable page.
Every vial size on one sheet, to keep next to the vials. Plus the free Peptide Starter Kit: the units cheat sheet, the calculator and the beginner masterclass.
This chart is one of 168 in the Reconstitution Encyclopedia: 67 peptides and blends, every common vial size, mixed step by step.
Questions
How much bacteriostatic water do you add to a 70 mg (10/10/50) GLOW vial?
In the Reconstitution Insights video, Matthew adds 3 mL. That makes 23.33 mg/mL, so each unit on a U-100 insulin syringe holds 233.3 mcg. You can use more or less water: the amount of peptide in the vial stays the same, only what each unit holds changes. The calculator works it out for any amount.
How many units do I draw for an amount of GLOW?
Divide the amount you want (in mcg) by what one unit holds. For example, 2.8 mg from the 70 mg (10/10/50) vial mixed with 3 mL is 12 units (0.12 mL). The chart above lists the other amounts.
What is a unit on an insulin syringe?
U-100 insulin syringes are marked in units: 100 units is 1 mL, so 1 unit is 0.01 mL whatever the size of the syringe. A 0.3 mL syringe holds 30 units and a 0.5 mL syringe holds 50.
Is this dosing advice?
No. The amounts are examples for the arithmetic, not recommendations. This page is for research and education only and isn't medical advice.
