How to reconstitute BPC-157 + TB-500
The BPC-157 + TB-500 blend, often called the Wolverine stack, comes as one vial holding equal amounts of both: 10 mg (5 mg + 5 mg) or 20 mg (10 mg + 10 mg). Because the two are mixed 1 to 1, every amount you draw is half of each.
- 10 mg (5/5) vial: add 2 mL of bacteriostatic water. That makes 5 mg/mL, so each unit on a U-100 insulin syringe holds 50 mcg, and 800 mcg is 16 units.
- 20 mg (10/10) vial: add 3 mL of bacteriostatic water. That makes 6.67 mg/mL, so each unit on a U-100 insulin syringe holds 66.7 mcg, and 800 mcg is 12 units.
Get this chart as a printable PDF
The amounts are examples for the arithmetic, not recommendations. For research and education only.
BPC-157 + TB-500 units chart
What each amount comes to on a 1 mL (U-100) insulin syringe, with the water used in the video. Using a different amount of water? The calculator works it out.
10 mg (5/5) vial + 2 mL bacteriostatic water
5 mg/mL concentration50 mcg in each unit
| Amount | Units (U-100) | Volume | Of which |
|---|---|---|---|
| 400 mcg | 8 units | 0.08 mL | 200 mcg BPC-157 200 mcg TB-500 |
| 500 mcg | 10 units | 0.1 mL | 250 mcg BPC-157 250 mcg TB-500 |
| 600 mcg | 12 units | 0.12 mL | 300 mcg BPC-157 300 mcg TB-500 |
| 700 mcg | 14 units | 0.14 mL | 350 mcg BPC-157 350 mcg TB-500 |
| 800 mcg | 16 units | 0.16 mL | 400 mcg BPC-157 400 mcg TB-500 |
| 900 mcg | 18 units | 0.18 mL | 450 mcg BPC-157 450 mcg TB-500 |
| 1 mg | 20 units | 0.2 mL | 500 mcg BPC-157 500 mcg TB-500 |
| 1.1 mg | 22 units | 0.22 mL | 550 mcg BPC-157 550 mcg TB-500 |
| 1.2 mg | 24 units | 0.24 mL | 600 mcg BPC-157 600 mcg TB-500 |
| 1.5 mg | 30 units | 0.3 mL | 750 mcg BPC-157 750 mcg TB-500 |
| 2 mg | 40 units | 0.4 mL | 1 mg BPC-157 1 mg TB-500 |
20 mg (10/10) vial + 3 mL bacteriostatic water
6.67 mg/mL concentration66.7 mcg in each unit
| Amount | Units (U-100) | Volume | Of which |
|---|---|---|---|
| 400 mcg | 6 units | 0.06 mL | 200 mcg BPC-157 200 mcg TB-500 |
| 500 mcg | 7.5 units | 0.075 mL | 250 mcg BPC-157 250 mcg TB-500 |
| 600 mcg | 9 units | 0.09 mL | 300 mcg BPC-157 300 mcg TB-500 |
| 700 mcg | 10.5 units | 0.105 mL | 350 mcg BPC-157 350 mcg TB-500 |
| 800 mcg | 12 units | 0.12 mL | 400 mcg BPC-157 400 mcg TB-500 |
| 900 mcg | 13.5 units | 0.135 mL | 450 mcg BPC-157 450 mcg TB-500 |
| 1 mg | 15 units | 0.15 mL | 500 mcg BPC-157 500 mcg TB-500 |
| 1.1 mg | 16.5 units | 0.165 mL | 550 mcg BPC-157 550 mcg TB-500 |
| 1.2 mg | 18 units | 0.18 mL | 600 mcg BPC-157 600 mcg TB-500 |
| 1.5 mg | 22.5 units | 0.225 mL | 750 mcg BPC-157 750 mcg TB-500 |
| 2 mg | 30 units | 0.3 mL | 1 mg BPC-157 1 mg TB-500 |
Step by step
The same steps as the video, in writing.
- Check the label.
The vial says how much powder is in it: 10 mg (5/5) or 20 mg (10/10). For a blend, the label gives the total and the split: BPC-157 + TB-500.
- Draw the water.
Use bacteriostatic water: 2 mL for the 10 mg (5/5) vial; 3 mL for the 20 mg (10/10) vial. If it's more than 1 mL, a 3 mL syringe does it in one draw.
- Add it slowly.
Let the water run down the inside wall of the vial, then swirl gently until the powder has dissolved. Don't shake it.
- Write the strength on the vial.
For the 10 mg (5/5) vial that's 5 mg/mL, or 50 mcg per unit, plus the date you mixed it.
- Find your amount.
Read the units from the chart, or put your own numbers in the calculator. Then check it backwards: units × mcg per unit should give the amount you wanted.
On Peptide Insights
The BPC-157 + TB-500 overview
For what BPC-157 + TB-500 is and what research has looked at, here is Matthew's full video on Peptide Insights, his main channel. 9:54 long.
Free printable PDF
Get the BPC-157 + TB-500 chart as a printable page.
Every vial size on one sheet, to keep next to the vials. Plus the reader kit: a lab math worksheet, a lab log and a COA checklist.
This chart is one of 168 in the Reconstitution Encyclopedia: 67 peptides and blends, every common vial size, mixed step by step.
Questions
How much bacteriostatic water do you add to a 10 mg (5/5) BPC-157 + TB-500 vial?
In the Reconstitution Insights video, Matthew adds 2 mL. That makes 5 mg/mL, so each unit on a U-100 insulin syringe holds 50 mcg. You can use more or less water: the amount of peptide in the vial stays the same, only what each unit holds changes. The calculator works it out for any amount.
How much bacteriostatic water do you add to a 20 mg (10/10) BPC-157 + TB-500 vial?
In the Reconstitution Insights video, Matthew adds 3 mL. That makes 6.67 mg/mL, so each unit on a U-100 insulin syringe holds 66.7 mcg. You can use more or less water: the amount of peptide in the vial stays the same, only what each unit holds changes. The calculator works it out for any amount.
How many units do I draw for an amount of BPC-157 + TB-500?
Divide the amount you want (in mcg) by what one unit holds. For example, 800 mcg from the 10 mg (5/5) vial mixed with 2 mL is 16 units (0.16 mL); 800 mcg from the 20 mg (10/10) vial mixed with 3 mL is 12 units (0.12 mL). The chart above lists the other amounts.
What is a unit on an insulin syringe?
U-100 insulin syringes are marked in units: 100 units is 1 mL, so 1 unit is 0.01 mL whatever the size of the syringe. A 0.3 mL syringe holds 30 units and a 0.5 mL syringe holds 50.
Is this dosing advice?
No. The amounts are examples for the arithmetic, not recommendations. This page is for research and education only and isn't medical advice.
