How to reconstitute NAD+
NAD+ usually comes as a freeze-dried powder in a 500 mg or 1000 mg vial. It isn't technically a peptide, but it's mixed and measured the same way. It's measured in milligrams, not micrograms, and mixing the two up is where most errors start.
- 500 mg vial: add 1 mL of bacteriostatic water. That makes 500 mg/mL, so each unit on a U-100 insulin syringe holds 5 mg, and 100 mg is 20 units.
- 1000 mg vial: add 2 mL of bacteriostatic water. That makes 500 mg/mL, so each unit on a U-100 insulin syringe holds 5 mg, and 100 mg is 20 units.
Get this chart as a printable PDF
The amounts are examples for the arithmetic, not recommendations. For research and education only.
Matthew’s NAD+ mixing video is coming to Reconstitution Insights.
NAD+ units chart
What each amount comes to on a 1 mL (U-100) insulin syringe, with the water used in the video. Using a different amount of water? The calculator works it out.
500 mg vial + 1 mL bacteriostatic water
500 mg/mL concentration5 mg in each unit
| Amount | Units (U-100) | Volume |
|---|---|---|
| 50 mg | 10 units | 0.1 mL |
| 100 mg | 20 units | 0.2 mL |
| 150 mg | 30 units | 0.3 mL |
| 200 mg | 40 units | 0.4 mL |
| 250 mg | 50 units | 0.5 mL |
| 300 mg | 60 units | 0.6 mL |
| 350 mg | 70 units | 0.7 mL |
| 400 mg | 80 units | 0.8 mL |
| 450 mg | 90 units | 0.9 mL |
| 500 mg | 100 units | 1 mL |
1000 mg vial + 2 mL bacteriostatic water
500 mg/mL concentration5 mg in each unit
| Amount | Units (U-100) | Volume |
|---|---|---|
| 50 mg | 10 units | 0.1 mL |
| 100 mg | 20 units | 0.2 mL |
| 150 mg | 30 units | 0.3 mL |
| 200 mg | 40 units | 0.4 mL |
| 250 mg | 50 units | 0.5 mL |
| 300 mg | 60 units | 0.6 mL |
| 350 mg | 70 units | 0.7 mL |
| 400 mg | 80 units | 0.8 mL |
| 450 mg | 90 units | 0.9 mL |
| 500 mg | 100 units | 1 mL |
Step by step
The same steps as the video, in writing.
- Check the label.
The vial says how much powder is in it: 500 mg or 1000 mg.
- Draw the water.
Use bacteriostatic water: 1 mL for the 500 mg vial; 2 mL for the 1000 mg vial. If it's more than 1 mL, a 3 mL syringe does it in one draw.
- Add it slowly.
Let the water run down the inside wall of the vial, then swirl gently until the powder has dissolved. Don't shake it.
- Write the strength on the vial.
For the 500 mg vial that's 500 mg/mL, or 5 mg per unit, plus the date you mixed it.
- Find your amount.
Read the units from the chart, or put your own numbers in the calculator. Then check it backwards: units × mg per unit should give the amount you wanted.
Free printable PDF
Get the NAD+ chart as a printable page.
Every vial size on one sheet, to keep next to the vials. Plus the reader kit: a lab math worksheet, a lab log and a COA checklist.
This chart is one of 168 in the Reconstitution Encyclopedia: 67 peptides and blends, every common vial size, mixed step by step.
Questions
How much bacteriostatic water do you add to a 500 mg NAD+ vial?
In the Reconstitution Insights video, Matthew adds 1 mL. That makes 500 mg/mL, so each unit on a U-100 insulin syringe holds 5 mg. You can use more or less water: the amount of peptide in the vial stays the same, only what each unit holds changes. The calculator works it out for any amount.
How much bacteriostatic water do you add to a 1000 mg NAD+ vial?
In the Reconstitution Insights video, Matthew adds 2 mL. That makes 500 mg/mL, so each unit on a U-100 insulin syringe holds 5 mg. You can use more or less water: the amount of peptide in the vial stays the same, only what each unit holds changes. The calculator works it out for any amount.
How many units do I draw for an amount of NAD+?
Divide the amount you want (in mg) by what one unit holds. For example, 100 mg from the 500 mg vial mixed with 1 mL is 20 units (0.2 mL); 100 mg from the 1000 mg vial mixed with 2 mL is 20 units (0.2 mL). The chart above lists the other amounts.
What is a unit on an insulin syringe?
U-100 insulin syringes are marked in units: 100 units is 1 mL, so 1 unit is 0.01 mL whatever the size of the syringe. A 0.3 mL syringe holds 30 units and a 0.5 mL syringe holds 50.
Is this dosing advice?
No. The amounts are examples for the arithmetic, not recommendations. This page is for research and education only and isn't medical advice.
